Cytogenetic and flow cytometric analysis of new allotetraploid forms derived from wild cotton species with A1 and G genomes

Sevara K. Arslanova * Institute of Genetics and Plant Experimental Biology, Academy of Sciences of the Republic of Uzbekistan, Tashkent Region, Kibray District, 102122, Uzbekistan , Dilrabo K. Ernazarova Institute of Genetics and Plant Experimental Biology, Academy of Sciences of the Republic of Uzbekistan, Tashkent Region, Kibray District, 102122, Uzbekistan , Samarkand State University named after Sharof Rashidov , Feruza U. Rafiyeva Institute of Genetics and Plant Experimental Biology, Academy of Sciences of the Republic of Uzbekistan, Tashkent Region, Kibray District, 102122, Uzbekistan , Abdulqahhor Kh. Toshpulatov Institute of Genetics and Plant Experimental Biology, Academy of Sciences of the Republic of Uzbekistan, Tashkent Region, Kibray District, 102122, Uzbekistan , Madina D. Kholova Chirchik Branch of Tashkent State Medical University , Institute of Genetics and Plant Experimental Biology, Academy of Sciences of the Republic of Uzbekistan, Tashkent Region, Kibray District, 102122, Uzbekistan , Barno B. Oripova Institute of Genetics and Plant Experimental Biology, Academy of Sciences of the Republic of Uzbekistan, Tashkent Region, Kibray District, 102122, Uzbekistan , Tashkent State Medical University , Faxriddin N. Kushanov Institute of Genetics and Plant Experimental Biology, Academy of Sciences of the Republic of Uzbekistan, Tashkent Region, Kibray District, 102122, Uzbekistan , National University of Uzbekistan
* Corresponding author: arslanovasevara87@gmail.com

DOI:

https://doi.org/10.66432/kdhxfr21

Keywords:

Cotton, Gossypium L., G. australe, hybridization, allopolyploidy, cytogenetics, chromosome, meiosis, mitosis.

Abstract

Wild Australian and Afro-Asian cotton species are characterized by their natural resistance to diseases and pests, serving as valuable genetic resources for the introgression of these beneficial traits into cultivated cotton varieties. This study presents the results of research aimed at determining the ploidy level of novel allopolyploid hybrid forms derived from wild Australian (Gossypium nelsonii) and Afro-Asian (G. herbaceum) cotton species using interspecific hybridization and experimental polyploidy methods. Cytogenetic analyses demonstrated that the interspecific F1 hybrids possess a diploid chromosome set (2n=2x=26), whereas the synthetic F1C hybrids developed via experimental polyploidy exhibited a multiplied chromosome set (2n=4x=52). Furthermore, flow cytometry analysis conclusively confirmed this chromosome doubling, showing that the nuclear DNA content doubled from an average value of - 12.8 in the diploid parental forms to 25.492 in the F1C allotetraploids. The study evaluated the interspecific crossability of species belonging to different genomic groups and successfully generated genetically stable synthetic allotetraploids. This demonstrates the feasibility of utilizing wild diploid species in genetic and breeding research.

Author Biographies

  • Feruza U. Rafiyeva, Institute of Genetics and Plant Experimental Biology, Academy of Sciences of the Republic of Uzbekistan, Tashkent Region, Kibray District, 102122, Uzbekistan

    Doctor of Biological Sciences, Leading Researcher at the Laboratory of Plant Genomic Studies, Institute of Genetics and Plant Experimental Biology, Academy of Sciences of the Republic of Uzbekistan.

  • Abdulqahhor Kh. Toshpulatov, Institute of Genetics and Plant Experimental Biology, Academy of Sciences of the Republic of Uzbekistan, Tashkent Region, Kibray District, 102122, Uzbekistan

    Junior Researcher at the Laboratory of Plant Genomic Studies, Institute of Genetics and Plant Experimental Biology, Academy of Sciences of the Republic of Uzbekistan.

  • Madina D. Kholova, Chirchik Branch of Tashkent State Medical University, Institute of Genetics and Plant Experimental Biology, Academy of Sciences of the Republic of Uzbekistan, Tashkent Region, Kibray District, 102122, Uzbekistan

    PhD in Biological Sciences, Senior Researcher at the Laboratory of Plant Genomic Studies, Institute of Genetics and Plant Experimental Biology, Academy of Sciences of the Republic of Uzbekistan.

  • Faxriddin N. Kushanov, Institute of Genetics and Plant Experimental Biology, Academy of Sciences of the Republic of Uzbekistan, Tashkent Region, Kibray District, 102122, Uzbekistan, National University of Uzbekistan

    Doctor of Biological Sciences, Professor at the Laboratory of Plant Genomic Studies, Institute of Genetics and Plant Experimental Biology, Academy of Sciences of the Republic of Uzbekistan.

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Published

28-08-2026

Issue

Vol. 1 No. 3 (2026)

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Original Research

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How to Cite

Cytogenetic and flow cytometric analysis of new allotetraploid forms derived from wild cotton species with A1 and G genomes. (2026). Journal of Genetics and Applied Biotechnology, 1(3), e2026042. https://doi.org/10.66432/kdhxfr21

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