Combining Ability Analysis of Yield and Its Component Traits in Upland Cotton (Gossypium hirsutum L.) Using a Diallel Mating Design

Khatir Ali * Department of Plant Breeding and Genetics, Faculty of Agriculture, University of Agriculture Faisalabad-38400, Pakistan , Muhammad Azam Khan Department of Plant Breeding and Genetics, Faculty of Agriculture, University of Agriculture Faisalabad-38400, Pakistan , Wajiha Khan Department of Plant Breeding and Genetics, Faculty of Agriculture, University of Agriculture Faisalabad-38400, Pakistan , Feruza Umidulloevna Rafieva Institute of Genetics and Plant Experimental Biology, Academy of Sciences of Uzbekistan, Tashkent, Uzbekistan , Haiqa Mustafa Department of Plant Breeding and Genetics, Faculty of Agriculture, University of Agriculture Faisalabad-38400, Pakistan
* Corresponding author: khatirali34521@gmail.com

DOI:

https://doi.org/10.66432/fvr21578

Keywords:

Diallel Analysis, Dominant, Non-Additive, Combining Ability, Reciprocal Effects, Heterosis

Abstract

Cotton (Gossypium hirsutum L.) is a valuable fiber crop that is of great economic importance. The genetic basis of the yield-related traits was investigated in this study in a full diallel mating design that involved four parental genotypes (AGC-2, MNH-998, RH-647 and Super Gold) and their twelve F1 hybrids. Characteristics such as plant height, monopod and sym pod branches, bolls per plant, boll weight, lint percentage and seed cotton yield were considered. ANOVA indicated that there was a significant genetic variation across genotypes. The integration of the ability analysis revealed that not only general combining ability (GCA) but also specific combining ability (SCA) played a significant role with non-additive gene action dominating the majority of traits. Some crosses such as AGC-2 × RH-647, AGC-2 × MNH-998 and MNH-998 × RH-647 had high SCA effects and heterotic potential. The existence of important reciprocal effects (RE) indicated the importance of maternal influence. These results indicate that cross breeding and using heterosis is an effective method in enhancing cotton production and fiber-related characteristics.

Author Biographies

  • Khatir Ali, Department of Plant Breeding and Genetics, Faculty of Agriculture, University of Agriculture Faisalabad-38400, Pakistan

    Department of Plant Breeding and Genetics, Faculty of Agriculture, University of Agriculture Faisalabad-38400, Pakistan

  • Muhammad Azam Khan, Department of Plant Breeding and Genetics, Faculty of Agriculture, University of Agriculture Faisalabad-38400, Pakistan

    Department of Plant Breeding and Genetics, Faculty of Agriculture, University of Agriculture Faisalabad-38400, Pakistan

  • Wajiha Khan, Department of Plant Breeding and Genetics, Faculty of Agriculture, University of Agriculture Faisalabad-38400, Pakistan

    Department of Plant Breeding and Genetics, Faculty of Agriculture, University of Agriculture Faisalabad-38400, Pakistan

  • Feruza Umidulloevna Rafieva, Institute of Genetics and Plant Experimental Biology, Academy of Sciences of Uzbekistan, Tashkent, Uzbekistan

    Institute of Genetics and Plant Experimental Biology, Academy of Sciences of Uzbekistan, Tashkent, Uzbekistan

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Published

2026-02-28

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Vol. 1 No. 1 (2026)

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Original Research

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How to Cite

Combining Ability Analysis of Yield and Its Component Traits in Upland Cotton (Gossypium hirsutum L.) Using a Diallel Mating Design. (2026). Journal of Genetics and Applied Biotechnology, 1(1), e2026013. https://doi.org/10.66432/fvr21578

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