Optimization of LD50 and Induction of Mutation Through EMS in Dalbergia sissoo
Keywords:
Dalbergia sissoo; Mutagenesis; EMS; LD50; leaf morphologyAbstract
Dalbergia sissoo is an economically important tree of the Indian subcontinent. In this study, three ethyl methanesulfonate (EMS) concentrations with four exposure times were applied to identify the optimum combination for the induction of mutations. The lethal dose (LD) estimated using germination percentage was not a suitable criterion, as the survival percentages reduced drastically. The survival percentage after 30 days to estimate LD. Among the LD25, LD50, and LD75, the LD50 was identified as the optimal criterion for the survival of mutants. The LD50 was observed with two combinations of treatments: 0.48% EMS dose for 12 hours and 1.39% EMS dose for 6 hours. The progressive increase in EMS concentrations reduced the survival percentage, indicating residual toxicity. 0.48% MS for 12 hours and 1.39% EMS for 6 hours were used to develop a mutagenized population. Among the affected traits, height, stem morphology, leaf shapes and leaf size was. The chlorophyll content was negatively affected due to EMS; a decrease to 9.7 was observed compared to the control of 42.01. When the collected data were analyzed using analysis of variance significant differences were found among all the traits studied. A decrease in survival rate was also observed after six months and again after one year. The study would improve our understanding of EMS-induced mutagenesis and its use in tree plants.
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